Download Animal Cell Culture and Technology (THE BASICS (Garland by Michael Butler PDF

By Michael Butler

This booklet is a superb source for college kids and starting medical reaseraches. it's a solid resource of easy equipment and methods for winning laboratory cellphone tradition methods. many of the present phone tradition tools are coated yet this assurance isn't very distinctive. back, very good for novices.

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Additional info for Animal Cell Culture and Technology (THE BASICS (Garland Science))

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2 Laminar flow cabinet (class II). Courtesy of Forma Scientifica genic material such as virus-producing human cell lines or tissue samples carrying known human pathogens. 5. Incubators Incubators are a basic necessity for maintaining a constant temperature during cell culture, usually at 37°C for mammalian cells. 3). These three parameters are essential for maintaining the consistency of culture conditions as well as preserving the viability of the cells. A slight Basic equipment and laboratory design 35 decrease in temperature from the optimal may slow the cell growth rate but an increase in temperature is likely to be far more detrimental to the cells.

This will include a description of a suitable laboratory environment in which to manipulate such cells and some of the basic laboratory equipment needed. 2. Laboratory design A cell culture laboratory should allow the sterile handling of cultured cells with a minimal level of contamination. The growth rate of bacterial or fungal cells is usually so much greater than that of mammalian cells that no level of contamination can be tolerated in an animal cell culture. However, it is impractical to design a laboratory that is totally free of potentially contaminating microorganisms.

And Dawson, M. (eds) Cell Culture Labfax, pp. 209–218. BIOS Scientific, Oxford. G. (1977) Sulphonated polystyrene as an optimal substratum for the adhesion and spreading of mesenchymal cells in monovalent and divalent saline solutions. J. Cell Physiol. 90:511–519. L. G. (1976) Stimulation of clonal growth of normal fibroblasts with substrata coated with basic polymers. J. Cell Biol. 71:727–734. A. G. (1981) The use of low-temperature subculturing and culture surfaces coated with basic polymers to reduce the requirement for serum macromolecules.

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