By W. Mächtle, M. D. Lechner (auth.), W. Borchard, A. Straatmann (eds.)
This quantity contains 20 contributions of the twelfth assembly on Analytical Ultracentrifugation from March 1-2, 2001 in Duisburg, Germany. quite a few fields of ultracentrifugation are lined touching on learn difficulties in biochemistry, biophysical chemistry and macromolecular chemistry in addition to interacting platforms. New investigations in regards to the sedimentation thought are provided. The part transition of gels is handled, as is the sedimentation-diffusion equilibrium of gels. One part includes the hydrodynamics of biopolymers.
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0-ll aliquot of the nanoparticle suspension was transferred to aluminium boats (Lu¨di, Flawil, Switzerland) and dried for 4 h at 100 °C; the mass of the particle residue was determined using a Supermicro balance (Sartorius, Go¨ttingen, Germany). 0, 150 mM NaCl; gradient volume 11 ml). 0 ml water were placed on top of the gradient and centrifuged for 90 min at 7,000 rpm (Beckman Spinco L7 ultracentrifuge, rotor SW 41Ti; 20 °C). 5 ml) were harvested using a peristaltic pump and a narrow tube, inserted from above to the bottom of the gradient.
5. The common part of the curves corresponds to the expected D1 and D2 values which are in a good agreement with the D) and D+quantities as evaluated previously by the independent method. These results conﬁrm the validity of the method and the approximations used. Conclusions The method was derived for treating the W-like boundary proﬁles obtained in the low-speed regime of analytical ultracentrifugation. The method yields characteristics of the heterogeneous polymer components: weight part and diﬀerence in the diﬀusion coeﬃcients.
The diﬀerences between the two veff values thus amount to 15 or 17% of the higher values. 829 ml/g. 022 ml/g and in the apolar one it is zero. (In the latter case, however, the eﬀective ionic strength is strongly reduced by ion pair formation, as shown by conductivity measurements. ) It should be noted that with the THF/chloroform mixture the plot of Mw,eﬀ versus solvent density is strongly nonlinear. As emphasized earlier , in such cases it is highly questionable whether the veff value determined from the zero crossover of the plot is applicable to other solvent compositions from the same solvent system, in particular to the corresponding pure solvents.